Coding

Part:BBa_K4716998:Design

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


NorR (PnorV regulator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1080


Design Notes

We use this protein coding sequence to express the regulator protein to the promoter PnorV, to control the downstrem gene's express based on the existence of NO molecules in environment.


Source

The sequence of this gene is directly taken from the following literature's supplementary materials. Reference: Xiaoyu J. Chen et al., (2021) Rational Design and Characterization of Nitric Oxide Biosensors in E. coli Nissle 1917 and Mini SimCells, ACS Synthetic Biology 2021, 10 (10), 2566-2578. doi: 10.1021/acssynbio.1c00223

References